A Complete Guide to Testing with Mira Fertility Plus

by Mar 17, 2021

Want to learn how to test with Mira? No worries, we’re here to walk you through the process. Please follow this guide!

Before you test with Mira

Setting up:

Download the Mira App (App Store or Google Play) before your Starter Kit arrives. Create an account, enter your period dates, and check suggestions. As you set up your Mira App, don’t forget to do the following:

  1. Allow notifications from the Mira App to receive notifications and reminders about testing days.
  2. Get familiar with the Mira App calendar screen, once you start testing, it will display green dots on the days you should test.

Mira AppBefore testing:

Here are a few frequently asked questions to help you better understand the process.

  • What do I need to know about my cycle lengths and testing? Depending on your cycle length, you may need to test every day after your period or every 2-3 days. Most women will ovulate between 2 and 19 days before their next period. If your Mira arrives toward the end of your cycle, simply start testing starting from the next one. Make sure you stock up on wands ahead of time too!
  • What are the best times to test? We recommend you test using your first morning urine. If you prefer testing during any other time of the day, you can do so. However, make sure you choose a specific time every day to be consistent.

Note: If you get a positive reading in the morning, test again within the next 6 hours to be absolutely sure. Remember, LH surges often occur throughout the day. After a positive result, ovulation commonly occurs within the next 48 hours.

  • What are your tips if I decide to test during the day? Make sure you limit fluid intake 2 hours prior to the test. Excessive fluid intake will affect the accuracy of the tests (diluting the LH concentration).
  • How many times per cycle do I need to test? You will need approximately 10-15 wands per cycle, depending on its length. As time passes, Mira will learn about your cycle and remind you about your testing days. During the beginning of each cycle, you will test approximately once a day around your fertile window and twice per day on ovulation day. Mira will adapt to your cycle and notify you to test only when it is truly needed.

Mira Analyzer

How to test accurately with Mira Fertility Plus

Once you’re ready to start testing with Mira, please follow our tips and guidelines to achieve accurate results.

How to connect your analyzer:

  1. Follow the instructions on the Mira App to start pairing.
  2. While pairing, make sure your Analyzer screen is on. It may take several attempts to complete the first pairing.
  3. If you’re having issues, try again in another room or an isolated area of your home. Too many Bluetooth devices may be competing for the signal.
  4. After tapping on “Connect Mira Analyzer” wait up to 15-20 seconds to connect.
  5. Keep an eye on your Analyzer for any error messages.
  6. On some phones, the Bluetooth signal will vary depending on direction and distance. Try putting your phone on top, below, or within different distances around the Analyzer while pairing.
  7. If you have a Samsung phone, turn on “Location” when pairing.
  8. If you have a connection issue, restart your phone and the Analyzer, or push the “reset” pinhole on the back of the Mira Analyzer and try again.

How to accurately test with Mira:

How to test with Mira

  1. Use a fresh urine sample, immediately after you collect it. *Waiting can make a big difference in the test results as hormones degrade quickly.
  2. Leave the test wand in the urine sample for 10-12 seconds. Sampling the urine for too long or not enough time may cause test errors.
  3. Do not shake the test wand after sampling the urine.
  4. Keep the sampling end of the test wand pointing down at all times. Do not lift the sampling end higher than the reading end of the wand when reversing the cap.
  5. Insert the test wand immediately into the Analyzer after sampling the urine.
  6. After sampling, keep the Analyzer on a flat and horizontal surface as you insert the test wand. Do not move or tilt the Analyzer during the 16-minute countdown.
  7. Once the reading is completed, the test wand cannot be reused. The Analyzer will show an old test result if an already-used wand is re-inserted.

Still have questions about how to test? Watch this video tutorial!

How to Use Mira Fertility App and How to Test with Mira Fertility Analyzer

Reading your Mira test:

After testing, you’ll need to read your results. It’s important to keep in mind that, as you test, the Mira Fertility Plus will start creating a trend as it picks up on your hormone levels and fertility—and will pinpoint your ovulation. So, don’t be alarmed if you have fluctuating hormone levels day after day, this is normal.

Here are a few questions we often receive to further guide you on reading your results:

  • My hormone levels fluctuate a lot, what do I do? If you’re seeing some odd numbers, don’t be alarmed. Hormone levels vary depending on the person and cycle. Also, the trend or your “hormone curve” is more important than the value of a single test.
  • How do I know when I’m ovulating? You will identify a sharp increase in your LH & estrogen levels. Once you identify this surge, this means you will ovulate within 12-24 hours and will mark your 6-day fertility window for TTC or TTA!

Here are examples of how LH & estrogen charts may vary:

LH and E3G peak at the same day
E3G peak at days before LH peak

E3G peak with no LH peak About reading your estrogen levels:

  • What is E3G? E3G is a metabolite of estrogen in the urine and stimulates an LH surge. The E3G level rises 1-3 days before an LH surge. Its rise often overlaps with the fertile window. Our E3G level fluctuates depending on the person and cycle. Normally, it will be around 20-100 pg/mL during the non-fertile period and will increase to 100-400+ close to ovulation.
  • What is the range of E3G? After you ovulate, E3G levels will drop, and there will be another surge in the luteal phase, which means your body is entering the non-fertile period.
  • What is a normal E3G range? In adult women with regular cycles, normal estradiol levels range anywhere from 15 to 350 picograms per milliliter (pg/mL). However, these levels can fluctuate during your period and reach as high as 800 pg/mL and still be considered normal.
  • What is the difference between E2 and E3G? The E3G in urine are metabolized products of the E2 in your blood and do not share the same unit. The unit of E3G is ng/ml, whereas the unit of E2 is pg/ml. However, they share the same increase and decrease patterns.
Live stream with Sylvia on the estrogen topic

About reading your LH levels:

  • If my LH surge is low, does that indicate weak ovulation? Not necessarily. Hormones fluctuate depending on the person and cycle. Always keep in mind: the overall trend is more important than the absolute value of a single test. When you ovulate, you should see a sudden increase in LH concentrations, although the peak value can vary greatly. This means you may not have to necessarily reach “high” to ovulate.
  • Is there a possibility that though LH surges, there is no ovulation? In most cases, people ovulate when there is an LH surge. Very rarely, some may have an anovulatory cycle, meaning the absence of ovulation. Nevertheless, your LH will still surge, as your ovary is “trying” to make it work, but for some reason, the egg cannot break out from the ovary and it is not released. This happens rarely but is found more frequently in people with PCOS.
  • I see a surge in the morning and it disappears in the afternoon, why? LH tends to increase and decrease very sharply. An entire surge can disappear within 24 hours. So, it is very normal to see a surge in the morning and having it disappear that same afternoon or the next day. Once you see the surge, you will ovulate in 12-24 hours—this marks the best timing for TTC or TTA.
  • You may be wondering – I have two luteinizing hormones (LH) peaks, does that mean I ovulate twice in one cycle? According to recent studies, there are three types of gradual onset (2-6 days) of LH surge. Some of them lead to multiple positive ovulation test results for more than one day. This is why Mira Fertility Tracker is better than over the counter ovulation tracking kits. Mira allows you to see your curve and recognize the highest peak based on the quantitative LH level.


What does the Fertility Score mean?

The Fertility Score helps you understand how fertile you are during a cycle:

  • 10 is often your ovulation day
  • 0 or 1 means low fertility

On average, ovulation occurs 14 days before the beginning of the next period, but this may not be accurate for every woman. Every body is different.

Using Mira for the first time: When you use Mira for the first time the app will predict your Fertility Score based on the cycle information you recorded in the app. The fertility score will then adjust based on each hormone test in the future. In order to stay consistent, it is essential that you test on the days marked with a green dot on the app’s calendar.

Troubleshooting your Mira Fertility Plus:

  • I am having a syncing issue, how do I fix it?  This situation is very rare. Try re-entering your period on the app or reset the Analyzer using a pin. If the issue persists, please reach out to [email protected] and we will be happy to help!
  • Why are pages on the new app not loading? This could be a connectivity issue. Try to use a different network, close, and reopen the app. If your internet connection is poor, you’ll need to troubleshoot your internet connection.
  • Why can’t I turn on my analyzer? Try resetting the analyzer using a pin and re-charging it. If the issue persists, kindly reach out with photos and videos to [email protected]. We are happy to help.

We hope this information has been enlightening for your fertility goals! If you’re in need of more insights regarding your data, results, and hormone levels, feel free to send an email to [email protected] or check our FAQ! We are happy to help 💚

Order Your Mira Plus Today

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